Silk-based multilayered angle-ply annulus fibrosus construct to recapitulate form and function of the intervertebral disc.

ABSTRACT: Recapitulation of the form and function of complex tissue organization using appropriate biomaterials impacts success in tissue engineering endeavors. The annulus fibrosus (AF) represents a complex, multilamellar, hierarchical structure consisting of collagen, proteoglycans, and elastic fibers. To mimic the intricacy of AF anatomy, a silk protein-based multilayered, disc-like angle-ply construct was fabricated, consisting of concentric layers of lamellar sheets. Scanning electron microscopy and fluorescence image analysis revealed cross-aligned and lamellar characteristics of the construct, mimicking the native hierarchical architecture of the AF. Induction of secondary structure in the silk constructs was confirmed by infrared spectroscopy and X-ray diffraction. The constructs showed a co

SUBMITTER: Bhunia BK 

PROVIDER: S-EPMC5776988 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature