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A growing microcolony can survive and support persistent propagation of virulent phages.

ABSTRACT: Bacteria form colonies and secrete extracellular polymeric substances that surround the individual cells. These spatial structures are often associated with collaboration and quorum sensing between the bacteria. Here we investigate the mutual protection provided by spherical growth of a monoclonal colony during exposure to phages that proliferate on its surface. As a proof of concept we exposed growing colonies of Escherichia coli to a virulent mutant of phage P1. When the colony consists of less than [Formula: see text]50,000 members it is eliminated, while larger initial colonies allow long-term survival of both phage-resistant mutants and, importantly, colonies of mostly phage-sensitive members. A mathematical model predicts that colonies formed solely by phage-sensitive bacteria can survive because the growth of bacteria throughout the colony exceeds the killing of bacteria on the surface and pinpoints how the critical colony size depends on key parameters in the phage infection cycle.

SUBMITTER: Eriksen RS

PROVIDER: S-EPMC5777033 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

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A growing microcolony can survive and support persistent propagation of virulent phages.

Eriksen Rasmus Skytte RS Svenningsen Sine L SL Sneppen Kim K Mitarai Namiko N

Proceedings of the National Academy of Sciences of the United States of America 20171219 2

Bacteria form colonies and secrete extracellular polymeric substances that surround the individual cells. These spatial structures are often associated with collaboration and quorum sensing between the bacteria. Here we investigate the mutual protection provided by spherical growth of a monoclonal colony during exposure to phages that proliferate on its surface. As a proof of concept we exposed growing colonies of <i>Escherichia coli</i> to a virulent mutant of phage P1. When the colony consists ...[more]

PMID: 29259110

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Project description:BackgroundAs aquaculture continues to expand globally, diseases caused by Vibrio species are becoming increasingly prevalent. Vibriosis encompasses a range of infections, which can lead to symptoms such as skin lesions, hemorrhaging, and high mortality rates in fish and shellfish, especially in high-density farming systems, resulting in significant economic losses. Simultaneously, the extensive use of antibiotics has fostered the emergence of antibiotic-resistant bacteria, exacerbated disease outbreaks, and complicated control measures. Phage therapy, which leverages bacteriophages as natural antibacterial agents, offers a promising eco-friendly alternative to the antibiotics used in aquaculture. This study aimed to evaluate the potential of two vibriophages for phage therapy in aquaculture.ResultsTwo virulent vibriophages, vB_VcaP_R24D and vB_VcaP_R25D, were isolated from aquaculture wastewater from seafood markets using Vibrio campbellii LMG 11216T as the host strain. The two vibriophages were identified based on their morphology, infection dynamics, host range, genomic features, lytic activity, and environmental stability. Both phages belong to the podovirus morphotype and exhibit a lytic life cycle characterised by a short latent period (< 10 min). Genomic analyses confirmed the absence of lysogenic genes, virulence factors, and antibiotic-resistance genes, thereby ensuring genetic safety. Additionally, both phages demonstrated high stability over a broad range of temperatures (4-45 °C) and pH (3-10). Lytic curve analyses further indicated a robust lytic efficiency during the logarithmic growth phase of the vibriophages.ConclusionsThese biological and genomic characteristics highlight the potential of vB_VcaP_R24D and vB_VcaP_R25D as effective biocontrol agents for mitigating vibriosis in aquaculture. Although this study demonstrates their narrow host range, the possibility of phage infection in other untested hosts cannot be entirely excluded. Furthermore, the findings offer valuable insights for future research on phage-host interactions and the development of phage cocktails to improve disease management in aquaculture systems.

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A growing microcolony can survive and support persistent propagation of virulent phages.

Publications

A growing microcolony can survive and support persistent propagation of virulent phages.

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