Cell-specific and region-specific transcriptomics in the multiple sclerosis model: Focus on astrocytes.

ABSTRACT: Changes in gene expression that occur across the central nervous system (CNS) during neurological diseases do not address the heterogeneity of cell types from one CNS region to another and are complicated by alterations in cellular composition during disease. Multiple sclerosis (MS) is multifocal by definition. Here, a cell-specific and region-specific transcriptomics approach was used to determine gene expression changes in astrocytes in the most widely used MS model, experimental autoimmune encephalomyelitis (EAE). Astrocyte-specific RNAs from various neuroanatomic regions were attained using RiboTag technology. Sequencing and bioinformatics analyses showed that EAE-induced gene expression changes differed between neuroanatomic regions when comparing astrocytes from spinal cord, cerebell

SUBMITTER: Itoh N 

PROVIDER: S-EPMC5777065 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature