Biogenesis of the bacterial cbb₃ cytochrome c oxidase: Active subcomplexes support a sequential assembly model.

ABSTRACT: The cbb₃ oxidase has a high affinity for oxygen and is required for growth of bacteria, including pathogens, in oxygen-limited environments. However, the assembly of this oxidase is poorly understood. Most cbb₃ are composed of four subunits: the catalytic CcoN subunit, the two cytochrome c subunits (CcoO and CcoP) involved in electron transfer, and the small CcoQ subunit with an unclear function. Here, we address the role of these four subunits in cbb₃ biogenesis in the purple bacterium Rubrivivax gelatinosus Analyses of membrane proteins from different mutants revealed the presence of active CcoNQO and CcoNO subcomplexes and also showed that the CcoP subunit is not essential for their assembly. However, CcoP was required

SUBMITTER: Durand A 

PROVIDER: S-EPMC5777255 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature