MicroRNA-7 upregulates death receptor 5 and primes resistant brain tumors to caspase-mediated apoptosis.

ABSTRACT:

Background

MicroRNAs (miRs) are known to play a pivotal role in tumorigenesis, controlling cell proliferation and apoptosis. In this study, we investigated the potential of miR-7 to prime resistant tumor cells to apoptosis in glioblastoma (GBM).

Methods

We created constitutive and regulatable miR-7 expression vectors and utilized pharmacological inhibition of caspases and genetic loss of function to study the effect of forced expression of miR-7 on death receptor (DR) pathways in a cohort of GBM with established resistance to tumor necrosis factor apoptosis inducing ligand (TRAIL) and in patient-derived primary GBM stem cell (GSC) lines. We engineered adeno-associated virus (AAV)-miR-7 and stem cell (SC) releasing secretable (S)-TRAIL and utilized real time in vivo imaging a

SUBMITTER: Bhere D 

PROVIDER: S-EPMC5777493 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature