Significance of intratissue estrogen concentration coupled with estrogen receptors levels in colorectal cancer prognosis.

ABSTRACT: Dysregulation of estrogen related pathways is implicated colorectal cancer (CRC) development. However, significance of intratissue concentration of estrone (E1) and 17β-estradiol (E2) in relation to estrogen receptor (ESR) expression level was not addressed so far. Herein, we measured E1 and E2 intratissue concentration using liquid chromatography electrospray ionization tandem mass spectrometry (ESI LC/MS) and mRNA levels of ESR1 and ESR2 using RT-qPCR in cancerous and histopathologically unchanged tissue from 75 and 110 CRC patients, respectively. The obtained results were associated with clinicopathological factors, expression of estrogen dependent genes (CTNNB1, CCND1) and prognostic significance. We found no statistically significant differences in E1 or E2 concentration

SUBMITTER: Rawluszko-Wieczorek AA 

PROVIDER: S-EPMC5777792 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature