Loss of organic cation transporter 3 (Oct3) leads to enhanced proliferation and hepatocarcinogenesis.

ABSTRACT:

Background

Organic cation transporters (OCT) are responsible for the uptake of a broad spectrum of endogenous and exogenous substrates. Downregulation of OCT is frequently observed in human hepatocellular carcinoma (HCC) and is associated with a poor outcome. The aim of our current study was to elucidate the impact of OCT3 on hepatocarcinogenesis.

Methods

Transcriptional and functional loss of OCT was investigated in primary murine hepatocytes, derived from Oct3-knockout (Oct3^-/-; FVB.Slc22a3^tm1Dpb ) and wildtype (WT) mice. Liver tumors were induced in Oct3^-/- and WT mice with Diethylnitrosamine and Phenobarbital over 10 months and characterized macroscopically and microscopically. Key survival pathways were investigated by Western Blo

SUBMITTER: Vollmar J 

PROVIDER: S-EPMC5777802 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature