Salmonella enterica Serovar Typhimurium Alters the Extracellular Proteome of Macrophages and Leads to the Production of Proinflammatory Exosomes.

ABSTRACT: Salmonella enterica serovar Typhimurium is a Gram-negative bacterium, which can invade and survive within macrophages. Pathogenic salmonellae induce the secretion of specific cytokines from these phagocytic cells and interfere with the host secretory pathways. In this study, we describe the extracellular proteome of human macrophages infected with S Typhimurium, followed by analysis of canonical pathways of proteins isolated from the extracellular milieu. We demonstrate that some of the proteins secreted by macrophages upon S Typhimurium infection are released via exosomes. Moreover, we show that infected macrophages produce CD63⁺ and CD9⁺ subpopulations of exosomes at 2 h postinfection. Exosomes derived from infected macrophages trigger the Toll-

SUBMITTER: Hui WW 

PROVIDER: S-EPMC5778363 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature