Influence of thermodynamically unfavorable secondary structures on DNA hybridization kinetics.

ABSTRACT: Nucleic acid secondary structure plays an important role in nucleic acid-nucleic acid recognition/hybridization processes, and is also a vital consideration in DNA nanotechnology. Although the influence of stable secondary structures on hybridization kinetics has been characterized, unstable secondary structures, which show positive ΔG° with self-folding, can also form, and their effects have not been systematically investigated. Such thermodynamically unfavorable secondary structures should not be ignored in DNA hybridization kinetics, especially under isothermal conditions. Here, we report that positive ΔG° secondary structures can change the hybridization rate by two-orders of magnitude, despite the fact that their hybridization obeyed second-order reaction kinetics. The temperature dep

SUBMITTER: Hata H 

PROVIDER: S-EPMC5778496 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature