The expression of glycerol facilitators from various yeast species improves growth on glycerol of Saccharomyces cerevisiae.

ABSTRACT: Glycerol is an abundant by-product during biodiesel production and additionally has several assets compared to sugars when used as a carbon source for growing microorganisms in the context of biotechnological applications. However, most strains of the platform production organism Saccharomyces cerevisiae grow poorly in synthetic glycerol medium. It has been hypothesized that the uptake of glycerol could be a major bottleneck for the utilization of glycerol in S. cerevisiae. This species exclusively relies on an active transport system for glycerol uptake. This work demonstrates that the expression of predicted glycerol facilitators (Fps1 homologues) from superior glycerol-utilizing yeast species such as Pachysolen tannophilus, Komagataella pastoris, Yarrowia

SUBMITTER: Klein M 

PROVIDER: S-EPMC5779717 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature