Development of a High-Resolution Multi-Locus Microsatellite Typing Method for Colletotrichum gloeosporioides.

ABSTRACT: Colletotrichum gloeosporioides is an economically important fungal pathogen causing substantial yield losses indifferent host plants. To understand the genetic diversity and molecular epidemiology of this fungus, we have developed a novel, high-resolution multi-locus microsatellite typing (MLMT) method. Bioinformatic analysis of C. gloeosporioides unannotated genome sequence yielded eight potential microsatellite loci, of which five, CG1 (GT)_n, CG2 (GT1)_n, CG3 (TC)_n, CG4 (CT)_n, and CG5 (CT1)_n were selected for further study based on their universal amplification potential, reproducibility, and repeat number polymorphism. The selected microsatellites were used to analyze 31 strains of C. gloeosporioides isolated fro

SUBMITTER: Mehta N 

PROVIDER: S-EPMC5780373 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature