Premature polyadenylation of MAGI3 is associated with diminished N⁶-methyladenosine in its large internal exon.

ABSTRACT: In cancer, tumor suppressor genes (TSGs) are frequently truncated, causing their encoded products to be non-functional or dominant-negative. We previously showed that premature polyadenylation (pPA) of MAGI3 truncates the gene, switching its functional role from a TSG to a dominant-negative oncogene. Here we report that MAGI3 undergoes pPA at the intron immediately downstream of its large internal exon, which is normally highly modified by N⁶-methyladenosine (m⁶A). In breast cancer cells that upregulate MAGI3 ^pPA , m⁶A levels in the large internal exon of MAGI3 are significantly reduced compared to cells that do not express MAGI3 ^pPA . We further find that MAGI3 ^pPA transcripts are significantly depleted of m⁶A mo

SUBMITTER: Ni TK 

PROVIDER: S-EPMC5780518 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature