Phased secondary small interfering RNAs in Panaxnotoginseng.

ABSTRACT:

Background

Recent results demonstrated that either non-coding or coding genes generate phased secondary small interfering RNAs (phasiRNAs) guided by specific miRNAs. Till now, there is no studies for phasiRNAs in Panax notoginseng (Burk.) F.H. Chen (P. notoginseng), an important traditional Chinese herbal medicinal plant species.

Methods

Here we performed a genome-wide discovery of phasiRNAs and its host PHAS loci in P. notoginseng by analyzing small RNA sequencing profiles. Degradome sequencing profile was used to identify the trigger miRNAs of these phasiRNAs and potential targets of phasiRNAs. We also used RLM 5'-RACE to validate some of the identified phasiRNA targets.

Results

After analyzing 24 small RNA sequencing profiles of P. notoginseng, 204 and 90 PHAS loci

SUBMITTER: Chen K 

PROVIDER: S-EPMC5780745 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature