Role of human Toll-like receptors in naturally occurring influenza A infections.

ABSTRACT:

Background

We investigated the roles of Toll-like receptors (TLRs) in naturally occurring influenza.

Methods

A prospective, case - control study was conducted. Adults hospitalized with virologically confirmed influenza A infections (onset <48 hours, before treatment) were compared with age-/gender-matched controls. TLRs (2, 3, 4, 7, 8, 9) expression in monocytes and dendritic cells (DCs - total, myeloid, plasmacytoid) was quantitated using flow cytometry. Gene expression of RLRs (RIG-1, MDA-5) was evaluated using real-time PCR. Concomitant signaling molecules expression, plasma cytokine/chemokine concentrations, and respiratory tract viral loads were measured. PBMCs were cultured and stimulated ex vivo with TLR-specific ligands for cytokine responses.

Results

Forty tw

SUBMITTER: Lee N 

PROVIDER: S-EPMC5781199 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature