IL-1β mediates lung neutrophilia and IL-33 expression in a mouse model of viral-induced asthma exacerbation.

ABSTRACT:

Background

Viral-induced asthma exacerbations, which exhibit both Th1-type neutrophilia and Th2-type inflammation, associate with secretion of Interleukin (IL)-1β. IL-1β induces neutrophilic inflammation. It may also increase Th2-type cytokine expression. We hypothesised that IL-1β is causally involved in both Th1 and Th2 features of asthma exacerbations. This hypothesis is tested in our mouse model of viral stimulus-induced asthma exacerbation.

Method

Wild-type (WT) and IL-1β deficient (IL-1β^-/-) mice received house dust mite (HDM) or saline intranasally during three weeks followed by intranasal dsRNA (PolyI:C molecule known for its rhinovirus infection mimic) for three consecutive days to provoke exacerbation. Bronchoalveolar lavage fluid was analysed for inflam

SUBMITTER: Mahmutovic Persson I 

PROVIDER: S-EPMC5781288 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature