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Genome-wide DNA Methylation analysis in response to salinity in the model plant caliph medic (Medicago truncatula).


ABSTRACT:

Background

DNA methylation has a potential role in controlling gene expression and may, therefore, contribute to salinity adaptation in plants. Caliph medic (Medicago truncatula) is a model legume of moderate salinity tolerance capacity; however, a base-resolution DNA methylome map is not yet available for this plant.

Results

In this report, a differential whole-genome bisulfite sequencing (WGBS) was carried out using DNA samples extracted from root tissues exposed to either control or saline conditions. Around 50 million differentially methylated sites (DMSs) were recognized, 7% of which were significantly (p < 0.05, FDR < 0.05) altered in response to salinity. This analysis showed that 77.0% of the contexts of DMSs were mCHH, while only 9.1% and 13.9% were mCHG and mCG, re

SUBMITTER: Yaish MW 

PROVIDER: S-EPMC5781308 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature