REST represses miR-124 and miR-203 to regulate distinct oncogenic properties of glioblastoma stem cells.

ABSTRACT:

Background

Glioblastoma (GBM) is one of the most common, aggressive, and invasive human brain tumors. There are few reliable mechanism-based therapeutic approaches for GBM patients. The transcriptional repressor RE1 silencing transcriptional factor (REST) regulates the oncogenic properties of a class of GBM stem-like cells (high-REST [HR]-GSCs) in humans. However, it has been unclear whether REST represses specific targets to regulate specific oncogenic functions or represses all targets with overlapping functions in GSCs.

Methods

We used genome-wide, biochemical, and mouse intracranial tumorigenic assays to identify and determine functions of microRNA (miR) targets of REST in 2 independent HR-GSC lines.

Results

Here we show that REST represses 2 major miR gene target

SUBMITTER: Marisetty AL 

PROVIDER: S-EPMC5781317 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature