APKC-mediated displacement and actomyosin-mediated retention polarize Miranda in Drosophila neuroblasts.

ABSTRACT: Cell fate assignment in the nervous system of vertebrates and invertebrates often hinges on the unequal distribution of molecules during progenitor cell division. We address asymmetric fate determinant localization in the developing Drosophila nervous system, specifically the control of the polarized distribution of the cell fate adapter protein Miranda. We reveal a step-wise polarization of Miranda in larval neuroblasts and find that Miranda's dynamics and cortical association are differently regulated between interphase and mitosis. In interphase, Miranda binds to the plasma membrane. Then, before nuclear envelope breakdown, Miranda is phosphorylated by aPKC and displaced into the cytoplasm. This clearance is necessary for the subsequent establishment of asymmetric Miranda localiz

SUBMITTER: Hannaford MR 

PROVIDER: S-EPMC5783611 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature