Identification of a potent MAR element from the human genome and assessment of its activity in stably transfected CHO cells.

ABSTRACT: Low-level and unstable transgene expression are common issues using the CHO cell expression system. Matrix attachment regions (MARs) enhance transgene expression levels, but additional research is needed to improve their function and to determine their mechanism of action. MAR-6 from CHO chromosomes actively mediates high and consistent gene expression. In this study, we compared the effects of two new MARs and MAR-6 on transgene expression in recombinant CHO cells and found one potent MAR element that can significantly increase transgene expression. Two MARs, including the human CSP-B MAR element and DHFR intron MAR element from CHO cells, were cloned and inserted downstream of the poly(A) site in a eukaryotic vector. The constructs were transfected into CHO cells, and the expression leve

SUBMITTER: Tian ZW 

PROVIDER: S-EPMC5783848 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature