New keratinolytic bacteria in valorization of chicken feather waste.

ABSTRACT: There is an increasing demand for cost-effective and ecologically-friendly methods for valorization of poultry feather waste, in which keratinolytic bacteria present a great potential. Feather-degrading bacteria were isolated from living poultry and a single strain, identified as Kocuria rhizophila p3-3, exhibited significant keratinolytic properties. The bacterial strain effectively degraded up to 52% of chicken feathers during 4 days of culture at 25 °C. Zymographic analysis revealed the presence of two dominating proteolytic enzymes in the culture fluid. Culture conditions were optimized in order to maximize the liberation of soluble proteins and free amino acids. A two-step procedure was used, comprising a Plackett-Burman screening design, followed by a Box-Behnken design. Concentratio

SUBMITTER: Laba W 

PROVIDER: S-EPMC5783986 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature