A new dry eye mouse model produced by exorbital and intraorbital lacrimal gland excision.

ABSTRACT: Chronic dry eye is an increasingly prevalent condition worldwide, with resulting loss of visual function and quality of life. Relevant, repeatable, and stable animal models of dry eye are still needed. We have developed an improved surgical mouse model for dry eye based on severe aqueous fluid deficiency, by excising both the exorbital and intraorbital lacrimal glands (ELG and ILG, respectively) of mice. After ELG plus ILG excision, dry eye symptoms were evaluated using fluorescein infiltration observation, tear production measurement, and histological evaluation of ocular surface. Tear production in the model mice was significantly decreased compared with the controls. The corneal fluorescein infiltration score of the model mice was also significantly increased compared with the controls.

SUBMITTER: Shinomiya K 

PROVIDER: S-EPMC5784089 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature