Variants of the 5'-terminal region of p53 mRNA influence the ribosomal scanning and translation efficiency.

ABSTRACT: The p53 protein is one of the major cell cycle regulators. The protein is expressed as at least twelve protein isoforms resulting from the use of alternative promoters, alternative splicing or downstream initiation codons. Importantly, there is growing evidence that translation initiation of p53 mRNA may be regulated by the structure and length of the naturally occurring variants of the 5'-terminal region of p53 mRNA transcripts. Here, several mRNA constructs were synthesized with variable length of the p53 5'-terminal regions and encoding luciferase reporter protein, and their translation was monitored continuously in situ in a rabbit reticulocyte lysate system. Moreover, four additional mRNA constructs were prepared. In two constructs, the structural context of AUG1 initiation codon was

SUBMITTER: Zydowicz-Machtel P 

PROVIDER: S-EPMC5784139 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature