Vertical Light Sheet Enhanced Side-View Imaging for AFM Cell Mechanics Studies.

ABSTRACT: The ability to measure dynamic structural changes within a cell under applied load is essential for developing more accurate models of cell mechanics and mechanotransduction. Atomic force microscopy is a powerful tool for evaluating cell mechanics, but the dominant applied forces and sample strains are in the vertical direction, perpendicular to the imaging plane of standard fluorescence imaging. Here we report on a combined sideways imaging and vertical light sheet illumination system integrated with AFM. Our system enables high frame rate, low background imaging of subcellular structural dynamics in the vertical plane synchronized with AFM force data. Using our system for cell compression measurements, we correlated stiffening features in the force indentation data with onset of nuclear

SUBMITTER: Beicker K 

PROVIDER: S-EPMC5784156 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature