Reversible swelling of SBMV is associated with reversible disordering.

ABSTRACT: The structures of the compact and swollen southern bean mosaic virus (SBMV) particles have been compared by X-ray diffraction and proton magnetic resonance (PMR). Small-angle X-ray scattering showed that removal of divalent cations at alkaline pH causes the particle diameter to increase from 289Å in the native SBMV by 12% in solution and by 9% in microcrystals. The swelling is fully reversible upon re-addition of Ca²⁺ and Mg²⁺ ions, as shown by the X-ray patterns at 6Å resolution and by the 270MHz PMR spectra. Beyond 30Å resolution, X-ray patterns from the compact SBMV in solution and in microcrystals show fine fringes of ∼1/225Å^-1 width extending to 6Å resolution, whereas patterns from the swollen SBMV in solution and in microcrystals show only broader fri

SUBMITTER: Li J 

PROVIDER: S-EPMC5784231 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature