BioID Reveals Novel Proteins of the Plasmodium Parasitophorous Vacuole Membrane.

ABSTRACT: During their development within the vertebrate host, Plasmodium parasites infect hepatocytes and red blood cells. Within these cells, parasites are surrounded by a parasitophorous vacuole membrane (PVM). The PVM plays an essential role for the interaction of parasites with their host cells; however, only a limited number of proteins of this membrane have been identified so far. This is partially because systematic proteomic analysis of the protein content of the PVM has been difficult in the past, due to difficulties encountered in attempts to separate the PVM from other membranes such as the parasite plasma membrane. In this study, we adapted the BioID technique to in vitro-cultivated Plasmodium berghei blood stage parasites and utilized the promiscuous biotin ligase

SUBMITTER: Schnider CB 

PROVIDER: S-EPMC5784244 | biostudies-literature | 2018 Jan-Feb

REPOSITORIES: biostudies-literature