Metabolic adaptation to intermittent fasting is independent of peroxisome proliferator-activated receptor alpha.

ABSTRACT: BACKGROUND:Peroxisome proliferator-activated receptor alpha (PPARA) is a major regulator of fatty acid oxidation and severe hepatic steatosis occurs during acute fasting in Ppara-null mice. Thus, PPARA is considered an important mediator of the fasting response; however, its role in other fasting regiments such as every-other-day fasting (EODF) has not been investigated. METHODS:Mice were pre-conditioned using either a diet containing the potent PPARA agonist Wy-14643 or an EODF regimen prior to acute fasting. Ppara-null mice were used to assess the contribution of PPARA activation during the metabolic response to EODF. Livers were collected for histological, biochemical, qRT-PCR, and Western blot analysis. RESULTS:Acute fasting activated PPARA and led to steatosis, whereas EODF protected

SUBMITTER: Li G 

PROVIDER: S-EPMC5784329 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature