Developmental dynamics of gene expression and alternative polyadenylation in the Caenorhabditis elegans germline.

ABSTRACT:

Background

The 3' untranslated regions (UTRs) of mRNAs play a major role in post-transcriptional regulation of gene expression. Selection of transcript cleavage and polyadenylation sites is a dynamic process that produces multiple transcript isoforms for the same gene within and across different cell types. Using LITE-Seq, a new quantitative method to capture transcript 3' ends expressed in vivo, we have characterized sex- and cell type-specific transcriptome-wide changes in gene expression and 3'UTR diversity in Caenorhabditis elegans germline cells undergoing proliferation and differentiation.

Results

We show that nearly half of germline transcripts are alternatively polyadenylated, that differential regulation of endogenous 3'UTR variants is common, and that alternative i

SUBMITTER: West SM 

PROVIDER: S-EPMC5784609 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature