Downregulation of HuR Inhibits the Progression of Esophageal Cancer through Interleukin-18.

ABSTRACT:

Purpose

The purpose of this study was to investigate the effect of human antigen R (HuR) downregulation and the potential target genes of HuR on the progression of esophageal squamous cell carcinoma (ESCC).

Materials and methods

In this study, a proteomics assay was used to detect the expression of proteins after HuR downregulation, and a luciferase assay was used to detect the potential presence of a HuR binding site on the 3'-untranslated region (3'-UTR) of interleukin 18 (IL-18). In addition, colony formation assay, MTT, EdU incorporation assay, Western blot, flow cytometry, immunohistochemistry, transwell invasion assay, and wound healing assay were used.

Results

In the present study, we found that the expression of both HuR protein and mRNA levels were higher in

SUBMITTER: Xu X 

PROVIDER: S-EPMC5784622 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature