Identification of protein lysine methylation readers with a yeast three-hybrid approach.

ABSTRACT:

Background

Protein posttranslational modifications (PTMs) occur broadly in the human proteome, and their biological outcome is often mediated indirectly by reader proteins that specifically bind to modified proteins and trigger downstream effects. Particularly, many lysine methylation sites among histone and nonhistone proteins have been characterized; however, the list of readers associated with them is incomplete.

Results

This study introduces a modified yeast three-hybrid system (Y3H) to screen for methyllysine readers. A lysine methyltransferase is expressed together with its target protein or protein domain functioning as bait, and a human cDNA library serves as prey. Proof of principle was established using H3K9me3 as a bait and known H3K9me3 readers like the chromodom

SUBMITTER: Rawluszko-Wieczorek AA 

PROVIDER: S-EPMC5784651 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature