PHENOS: a high-throughput and flexible tool for microorganism growth phenotyping on solid media.

ABSTRACT:

Background

Microbial arrays, with a large number of different strains on a single plate printed with robotic precision, underpin an increasing number of genetic and genomic approaches. These include Synthetic Genetic Array analysis, high-throughput Quantitative Trait Loci (QTL) analysis and 2-hybrid techniques. Measuring the growth of individual colonies within these arrays is an essential part of many of these techniques but is useful for any work with arrays. Measurement is typically done using intermittent imagery fed into complex image analysis software, which is not especially accurate and is challenging to use effectively. We have developed a simple and fast alternative technique that uses a pinning robot and a commonplace microplate reader to continuously measure the thickne

SUBMITTER: Barton DBH 

PROVIDER: S-EPMC5784713 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature