The Profile and Dynamics of RNA Modifications in Animals.

ABSTRACT: More than a hundred distinct modified nucleosides have been identified in RNA, but little is known about their distribution across different organisms, their dynamic nature and their response to cellular and environmental stress. Mass-spectrometry-based methods have been at the forefront of identifying and quantifying modified nucleosides. However, they often require synthetic reference standards, which do not exist in the case of many modified nucleosides, and this therefore impedes their analysis. Here we use a metabolic labelling approach to achieve rapid generation of bio-isotopologues of the complete Caenorhabditis elegans transcriptome and its modifications and use them as reference standards to characterise the RNA modification profile in this multicellular organism through an untar

SUBMITTER: van Delft P 

PROVIDER: S-EPMC5784800 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature