ATP Evokes Ca²⁺ Responses and CXCL5 Secretion via P2X₄ Receptor Activation in Human Monocyte-Derived Macrophages.

ABSTRACT: Leukocytes sense extracellular ATP, a danger-associated molecular pattern, released during cellular stress and death, via activation of cell surface P2X and P2Y receptors. Here, we investigate P2 receptor expression in primary human monocyte-derived macrophages and receptors that mediate ATP-evoked intracellular [Ca²⁺]_i signals and cytokine production in response to ATP concentrations that exclude P2X₇ receptor activation. Expression of P2X₁, P2X₄, P2X₅, P2X₇, P2Y₁, P2Y₂, P2Y₄, P2Y₆, P2Y₁₁, and P2Y₁₃ was confirmed by quantitative RT-PCR and immunocytochemistry. ATP elicited intracellular Ca²⁺ responses in a concentration-dependent fashion

SUBMITTER: Layhadi JA 

PROVIDER: S-EPMC5784824 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature