A novel PCR-based system for the detection of four species of human malaria parasites and Plasmodium knowlesi.

ABSTRACT: A microscopy-based diagnosis is the gold standard for the detection and identification of malaria parasites in a patient's blood. However, the detection of cases involving a low number of parasites and the differentiation of species sometimes requires a skilled microscopist. Although PCR-based diagnostic methods are already known to be very powerful tools, the time required to apply such methods is still much longer in comparison to traditional microscopic observation. Thus, improvements to PCR systems are sought to facilitate the more rapid and accurate detection of human malaria parasites Plasmodium falciparum, P. vivax, P. ovale, and P. malariae, as well as P. knowlesi, which is a simian malaria parasite that is currently widely distributed in Southeast Asia. A nested PCR that targets t

SUBMITTER: Komaki-Yasuda K 

PROVIDER: S-EPMC5785027 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature