The 3'-untranslated region of mRNAs as a site for ribozyme cleavage-dependent processing and control in bacteria.

ABSTRACT: Besides its primary informational role, the sequence of the mRNA (mRNA) including its 5'- and 3'- untranslated regions (UTRs), contains important features that are relevant for post-transcriptional and translational regulation of gene expression. In this work a number of bacterial twister motifs are characterized both in vitro and in vivo. The analysis of their genetic contexts shows that these motifs have the potential of being transcribed as part of polycistronic mRNAs, thus we suggest the involvement of bacterial twister motifs in the processing of mRNA. Our data show that the ribozyme-mediated cleavage of the bacterial 3'-UTR has major effects on gene expression. While the observed effects correlate weakly with the kinetic parameters of the ribozymes, they show dependence on motif-spec

SUBMITTER: Felletti M 

PROVIDER: S-EPMC5785228 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature