Live imaging analysis of human gastric epithelial spheroids reveals spontaneous rupture, rotation and fusion events.

ABSTRACT: Three-dimensional cultures of primary epithelial cells including organoids, enteroids and epithelial spheroids have become increasingly popular for studies of gastrointestinal development, mucosal immunology and epithelial infection. However, little is known about the behavior of these complex cultures in their three-dimensional culture matrix. Therefore, we performed extended time-lapse imaging analysis (up to 4 days) of human gastric epithelial spheroids generated from adult tissue samples in order to visualize the dynamics of the spheroids in detail. Human gastric epithelial spheroids cultured in our laboratory grew to an average diameter of 443.9 ± 34.6 μm after 12 days, with the largest spheroids reaching diameters of >1000 μm. Live imaging analysis revealed that spheroid growth was a

SUBMITTER: Sebrell TA 

PROVIDER: S-EPMC5785432 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature