High-resolution spatiotemporal transcriptome mapping of tomato fruit development and ripening.

ABSTRACT: Tomato (Solanum lycopersicum) is an established model for studying fruit biology; however, most studies of tomato fruit growth and ripening are based on homogenized pericarp, and do not consider the internal tissues, or the expression signatures of individual cell and tissue types. We present a spatiotemporally resolved transcriptome analysis of tomato fruit ontogeny, using laser microdissection (LM) or hand dissection coupled with RNA-Seq analysis. Regulatory and structural gene networks, including families of transcription factors and hormone synthesis and signaling pathways, are defined across tissue and developmental spectra. The ripening program is revealed as comprising gradients of gene expression, initiating in internal tissues then radiating outward, and basipetally along a latitu

SUBMITTER: Shinozaki Y 

PROVIDER: S-EPMC5785480 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature