Biologically active constituents of the secretome of human W8B2⁺ cardiac stem cells.

ABSTRACT: The benefits of adult stem cells for repair of the heart have been attributed to the repertoire of salutary paracrine activities they appear to exert. We previously isolated human W8B2⁺ cardiac stem cells (CSCs) and found they powerfully influence cardiomyocytes and endothelial cells to collectively promote cardiac repair and regeneration. Here, the complexity of the W8B2⁺ CSC secretomes was characterised and examined in more detail. Using ion exchange chromatography to separate soluble proteins based on their net surface charge, the secreted factors responsible for the pro-survival activity of W8B2⁺ CSCs were found within the low and medium cation fractions. In addition to the soluble proteins, extracellular vesicles generated from W8B2⁺ CSCs no

SUBMITTER: Nie S 

PROVIDER: S-EPMC5785502 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature