Differential Tolerance to Lead and Cadmium of Micropropagated Gypsophila fastigiata Ecotype.

ABSTRACT: In vitro techniques may provide a suitable tool for effective propagation and conservation of plant species representing various ecological niches. The elaboration of such protocols is also prerequisite for selection of heavy-metal-tolerant plant material that could be afterwards used for restoration or remediation of polluted sites. In this study, culture protocol for Gypsophila fastigiata propagation was developed. The highest multiplication coefficient, which reached 6.5, and the best growth parameters were obtained on modified MS medium supplemented with 1.0 mg L-1 2iP and 0.2 mg L-1 IAA. The obtained cultures were treated with different concentrations of lead nitrate (0.1, 0.5, and 1.0 mM Pb(NO3)2) or cadmium chloride (0.5, 2.5, and 5.0 μM CdCl2). The growth parameters, photosynthetic

SUBMITTER: Muszynska E 

PROVIDER: S-EPMC5785615 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature