A COUP-TFII Human Embryonic Stem Cell Reporter Line to Identify and Select Atrial Cardiomyocytes.

ABSTRACT: Reporter cell lines have already proven valuable in identifying, tracking, and purifying cardiac subtypes and progenitors during differentiation of human pluripotent stem cells (hPSCs). We previously showed that chick ovalbumin upstream promoter transcription factor II (COUP-TFII) is highly enriched in human atrial cardiomyocytes (CMs), but not ventricular. Here, we targeted mCherry to the COUP-TFII genomic locus in hPSCs expressing GFP from the NKX2.5 locus. This dual atrial NKX2.5^EGFP/+-COUP-TFII^mCherry/+ reporter line allowed identification and selection of GFP⁺ (G⁺)/mCherry⁺ (M⁺) CMs following cardiac differentiation. These cells exhibited transcriptional and functional properties of atrial CMs, whereas G⁺/M

SUBMITTER: Schwach V 

PROVIDER: S-EPMC5785710 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature