Dual regulation of activity and intracellular localization of the PASTA kinase PrkC during Bacillus subtilis growth.

ABSTRACT: The activity of the PrkC protein kinase is regulated in a sophisticated manner in Bacillus subtilis cells. In spores, in the presence of muropeptides, PrkC stimulates dormancy exit. The extracellular region containing PASTA domains binds peptidoglycan fragments to probably enhance the intracellular kinase activity. During exponential growth, the cell division protein GpsB interacts with the intracellular domain of PrkC to stimulate its activity. In this paper, we have reinvestigated the regulation of PrkC during exponential and stationary phases. We observed that, during exponential growth, neither its septal localization nor its activity are influenced by the addition of peptidoglycan fragments or by the deletion of one or all PASTA domains. However, Dynamic Light Scattering experiments s

SUBMITTER: Pompeo F 

PROVIDER: S-EPMC5786024 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature