Improving the baculovirus expression vector system with vankyrin-enhanced technology.

ABSTRACT: The baculovirus expression vector system (BEVS) is a widely used platform for the production of recombinant eukaryotic proteins. However, the BEVS has limitations in comparison to other higher eukaryotic expression systems. First, the insect cell lines used in the BEVS cannot produce glycoproteins with complex-type N-glycosylation patterns. Second, protein production is limited as cells die and lyse in response to baculovirus infection. To delay cell death and lysis, we transformed several insect cell lines with an expression plasmid harboring a vankyrin gene (P-vank-1), which encodes an anti-apoptotic protein. Specifically, we transformed Sf9 cells, Trichoplusia ni High Five^TM cells, and SfSWT-4 cells, which can produce glycoproteins with complex-type N-glycosylation patterns.

SUBMITTER: Steele KH 

PROVIDER: S-EPMC5786172 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature