The Role of ERK1/2 Activation in Sarpogrelate-Mediated Neuroprotection.

ABSTRACT:

Purpose

To characterize the mediators of 5-HT2A serotonin receptor-driven retinal neuroprotection.

Methods

Albino mice were treated intraperitoneally with saline or sarpogrelate, a 5-HT2A antagonist, immediately before light exposure (LE). Following LE, retinas were harvested for a high-throughput phosphorylation microarray to quantify activated phosphorylated proteins in G protein-coupled receptor (GPCR) signaling. To confirm microarray results and define temporal changes, Western blots of select GPCR signaling proteins were performed. Since both methodologies implicated MAPK/ERK activation, the functional significance of sarpogrelate-mediated ERK1/2 activation was examined by inhibition of ERK1/2 phosphorylation via pretreatment with the MEK inhibitor (MEKi) PD0325901. The

SUBMITTER: Ku CA 

PROVIDER: S-EPMC5786286 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature