Systematic analysis of ribophagy in human cells reveals bystander flux during selective autophagy.

ABSTRACT: Ribosomes are abundant cellular machines ^1,2 that are regulated by assembly, supernumerary subunit turnover and nascent chain quality control mechanisms ^1-5 . Moreover, nitrogen starvation in yeast has been reported to promote selective ribosome delivery to the vacuole in an autophagy conjugation system dependent manner, a process called 'ribophagy' ^6,7 . However, whether ribophagy in mammals is selective or regulated is unclear. Using Ribo-Keima flux reporters, we find that starvation or mTOR inhibition promotes VPS34-dependent ribophagic flux, which, unlike yeast, is largely independent of ATG8 conjugation and occurs concomitantly with other cytosolic protein autophagic flux reporters ^8,9 . Ribophagic flux was not induced upon inhibition of tra

SUBMITTER: An H 

PROVIDER: S-EPMC5786475 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature