Multi-Platform Sequencing Approach Reveals a Novel Transcriptome Profile in Pseudorabies Virus.

ABSTRACT: Third-generation sequencing is an emerging technology that is capable of solving several problems that earlier approaches were not able to, including the identification of transcripts isoforms and overlapping transcripts. In this study, we used long-read sequencing for the analysis of pseudorabies virus (PRV) transcriptome, including Oxford Nanopore Technologies MinION, PacBio RS-II, and Illumina HiScanSQ platforms. We also used data from our previous short-read and long-read sequencing studies for the comparison of the results and in order to confirm the obtained data. Our investigations identified 19 formerly unknown putative protein-coding genes, all of which are 5' truncated forms of earlier annotated longer PRV genes. Additionally, we detected 19 non-coding RNAs, including 5' and 3' t

SUBMITTER: Moldovan N 

PROVIDER: S-EPMC5786565 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature