Isolation and culture of primary adult skin fibroblasts from the Asian elephant (Elephas maximus).

ABSTRACT:

Background

Primary cultures from Asian elephants (Elephas maximus) allow scientists to obtain representative cells that have conserved most of their original characteristics, function, physiology and biochemistry. This technique has thus gained significant importance as a foundation for further cellular, cell biology and molecular research. Therefore, the aim of this study was to describe conditions for the successful establishment of primary adult fibroblasts from Asian elephant carcasses.

Methods

Ear tissue sample collection from Asian elephant carcasses and our recommendations are given. We describe here a simple modified protocol for successful isolation and maintenance of primary adult fibroblasts from elephant ear skin. Ear samples from each individual (five 3 ×

SUBMITTER: Siengdee P 

PROVIDER: S-EPMC5786883 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature