Rice nucleosome patterns undergo remodeling coincident with stress-induced gene expression.

ABSTRACT:

Background

Formation of nucleosomes along eukaryotic DNA has an impact on transcription. Major transcriptional changes occur in response to low external phosphate (Pi) in plants, but the involvement of chromatin-level mechanisms in Pi starvation responses have not been investigated.

Results

We mapped nucleosomes along with transcriptional changes after 24-h of Pi starvation in rice (Oryza sativa) by deep sequencing of micrococcal nuclease digested chromatin and ribosome-depleted RNA. We demonstrated that nucleosome patterns at rice genes were affected by both cis- and trans-determinants, including GC content and transcription. Also, categorizing rice genes by nucleosome patterns across the transcription start site (TSS) revealed nucleosome patterns that correlated with disti

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC5787291 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature