Defining murine organogenesis at single-cell resolution reveals a role for the leukotriene pathway in regulating blood progenitor formation.

ABSTRACT: During gastrulation, cell types from all three germ layers are specified and the basic body plan is established ¹ . However, molecular analysis of this key developmental stage has been hampered by limited cell numbers and a paucity of markers. Single-cell RNA sequencing circumvents these problems, but has so far been limited to specific organ systems ² . Here, we report single-cell transcriptomic characterization of >20,000 cells immediately following gastrulation at E8.25 of mouse development. We identify 20 major cell types, which frequently contain substructure, including three distinct signatures in early foregut cells. Pseudo-space ordering of somitic progenitor cells identifies dynamic waves of transcription and candidate regulators, which are validated by molec

SUBMITTER: Ibarra-Soria X 

PROVIDER: S-EPMC5787369 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature