Pathway-focused PCR array profiling of CAL-27 cell with over-expressed ZNF750.

ABSTRACT: Zinc-finger protein 750 (ZNF750) is the potential anti-cancer gene in oral squamous cell carcinoma (OSCC). The present study was to investigate the expression changes of ZNF750 in OSCC tissue and to reveal the induction of altered mRNA expression profiles caused by over-expressed ZNF750 in CAL-27 cell. The expression level of ZNF750 in tissue specimens from OSCC patients was detected by immunohistochemistry. Gene expression profiling was performed using Human Signal Transduction PathwayFinder RT² Profiler™ PCR Array. The expression changes of 84 key genes representing 10 signal transduction pathways in human following over-expressed ZNF750 in CAL-27 cell was examined. The expression of ZNF750 protein was reduced in OSCC tissues. The R² PCR Array analysis revealed that

SUBMITTER: Pan L 

PROVIDER: S-EPMC5787490 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature