Cell-specific responses to the cytokine TGFβ are determined by variability in protein levels.

ABSTRACT: The cytokine TGFβ provides important information during embryonic development, adult tissue homeostasis, and regeneration. Alterations in the cellular response to TGFβ are involved in severe human diseases. To understand how cells encode the extracellular input and transmit its information to elicit appropriate responses, we acquired quantitative time-resolved measurements of pathway activation at the single-cell level. We established dynamic time warping to quantitatively compare signaling dynamics of thousands of individual cells and described heterogeneous single-cell responses by mathematical modeling. Our combined experimental and theoretical study revealed that the response to a given dose of TGFβ is determined cell specifically by the levels of defined signaling proteins. This heter

SUBMITTER: Strasen J 

PROVIDER: S-EPMC5787704 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature