Authentic CRAC channel activity requires STIM1 and the conserved portion of the Orai N terminus.

ABSTRACT: Calcium (Ca²⁺) is an essential second messenger required for diverse signaling processes in immune cells. Ca²⁺ release-activated Ca²⁺ (CRAC) channels represent one main Ca²⁺ entry pathway into the cell. They are fully reconstituted via two proteins, the stromal interaction molecule 1 (STIM1), a Ca²⁺ sensor in the endoplasmic reticulum, and the Ca²⁺ ion channel Orai in the plasma membrane. After Ca²⁺ store depletion, STIM1 and Orai couple to each other, allowing Ca²⁺ influx. CRAC-/STIM1-mediated Orai channel currents display characteristic hallmarks such as high Ca²⁺ selectivity, an increase in current density when switching from a Ca²⁺-containing solution to a divalent-free Na

SUBMITTER: Derler I 

PROVIDER: S-EPMC5787803 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature